Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant Klebsiella pneumoniae isolate

摘要

Objective: To analyze the molecular mechanism of integron mediated multi-resistance in an ESBL-producing K. pneumoniae NJ 12 isolate. Methods: Susceptibility test was carried out by Kirby-Bauer method. Class Ⅰ , Ⅱ and Ⅲ integrons were detected by integrase gene PCR with primers that annealed to conserved regions of integron-encoded integrase genes intI1, intI2 and intI3. The variable region of integron was amplified by integron PCR with primers that targeted the conserved flanking regions, and the PCR product was sequenced. Six aminoglycoside modifying-enzyme genes, including ant(2″)-Ⅰ , ant(3″)-Ⅰ , aac(3)-Ⅰ , aac(3)-Ⅱ , aac (6′)-Ⅰ , and aac (6′)-Ⅱ , were detected. Results: K. pneumoniae NJ 12 was resistant to nine antibiotics, including piperacillin, ampicillin, cefuroxime, ceftazidime, cefotaxime, aztreonam, streptomycin, gentamicin and amikacin. This isolate was shown that there was positive with class I integron, ant(2″)-Ⅰ , ant(3″)-Ⅰ , aac(3)-Ⅱ andaac(6′)-Ⅰ modifying-enzyme genes. Neither class Ⅱ nor Ⅲ integron was detected; DNA sequencing of the fragment amplified by integron PCR revealed a novel cassette array aadB-cat-bla_(oxa-10/) aadAl. Conclusion: Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant K. pneumoniae NJ 12 isolate was reported from Nanjing area of China, with the GenBank accession number DQ141319.