Adaptive Responses of Escherichia coli for Oxidative and Protein Damage Using Bioluminescence Reporters

摘要

The recombinant bioluminescent Escherichia coli strains, DPD2511 and TV1061 containing the katG and grpE promoters, respectively, from Vibrio fischen fused to luxCDABE, were used to detect the adaptive and repair responses to oxidative damage caused by hydrogen peroxide (H_2O_2), and protein damage due to phenol. The response ratio, represented as the bioluminescence induced in subsequent inductions of DPD2511 and TV1061 with the mother cells previously induced by each chemical, i.e., H_2O_2 and phenol during the previous induction stage, decreased suddenly compared with the ratio of the control culture of each strain, meaning there is a possible adaptive response to stress caused by chemicals. Protein damage due to phenol was completely repaired by the second culturing after the initial induction, as was oxidative damage caused by H_2O_2 which was also rapidly repaired, as detected by the recovery of bioluminescence level. This result suggests that E, coli promptly adapt and repair oxidative and protein damage by H_2O_2 and phenol completely.