Macroporous chitin affinity membranes for wheat germ agglutinin purification from wheat germ

摘要

Marcroporous chitin membranes of controlled porosity and pore sizes have been prepared. They have good mechanical propertis and allow high flow rates of protein solutions at low pressure drops. Because of the numerous N-acetyl-D-glucosamine (GlcNAc) moieties they contain, the chitin membranes can be used for the separation of some valuable proteins both as affinity ligands and support matrix, without further modification. Due to their high porosity and high adsorption surface area, the chitin membranes provide a larger number of accessible binding sites for the wheat germ agglutinin than the chitin beads do.