A membrane based reactor with an enzyme immobilied by an avidin-biotion moloeulr recognition in polymer matrix

摘要

Glucose oxidase (GOD) and peroxidase (POD) have successfully been immobilized in a polypyrrole matrix by an avidinbiotin molecular recognition process. A biotin labeled pyrrole was the precursor of the electropolymerized polymer. The amount of enzyme entrapped in the polymeric matrix was 60/100 of the initial amount for GOD and 83/100 for POD; these values are higher than those previously reported for enzyme entrapment in polymer matrixes.