Colocalization of SOM and NOS in the neurons of raphe nuclei inner- vating the pharyngeal muscles in the rats: PRV, SOM and NADPH-d triple labeling study

摘要

Objective: to investigate the colocalization of Somatostatin (SOM) and NADPH-diaphorase (NADPH- d) of the neurons in raphe nuclei innervating pharyngeal muscles in the rats. Methods: After PRV was injected in- to the pharyngeal muscles, PRV and SOM immunofluoresecne double labeling procedure was completed at first, then proceeded NADPH-d histochemistry. Results: PRV and SOM double-labled cells were present mainly in the nucleus raphe magnus, but some PRV and SOM double-labled neurons were found in the other raphe nuclei as well, such as nucleus raphe pallidus, nucleus raphe obscurus, median raphe nucleus and dorsal raphe nucleus.