Construction of a vaccinia virus vectored multi-epitope live vaccine candidate for Plasmodium falciparum

摘要

To construct live recombinant vaccinina virus, the HGFSP gene encoding CSP, MSA1, MSA2, RESA, IL-1 and TT epitopes was inserted into the Eco RI and Ban HI sites of pSK plasmid. After digested with Eco RI, Bluntly ended by Klenow enzyme and digested with Sac I, the HGFSP gene was cloned into the Sma I and Sac I Sites of the vaccinia virus insertion vector (pJ2-16). Recombinant plasmids were identifed by gel electrophoresis, Restriction enzyme and enzyme map.