The effect of anastrozole on mRNA expression of oestrogen related gene in MCF-7 breast cancer cells

摘要

Objective: To look for additional markers of molecular biology response to anastrozole, a new aromatase inhibitor, on the growth and mRNA expression level of MCF-7 cell. Methods: We investigated the effect of anastrzole on growth and gene expression in the human breast cancer cell line MCF-7 and compared with the most widely used antiestrogen tamoxifen. We chose 4 genes to examine regulation of gene expression of estrogen regulated genes: PR A, PR B, ErbB-2 and cyclin D_1. Results: Compared with the tamoxifen, a statistically significant growth inhibition was observed with anastrozole. The PR A, PR B and cyclin D_1 mRNA level in anastrozole treated cells was sigificantly below the level in tamoxifen treated cells (P < 0.05). They had agonistic effect on ErbB gene (P > 0.05). Conclusion: The third generation of aromatase inhibitors anastrozole exert more inhibit function in some expression of estrogen regulated genes than tomoxifen in MCF-7 cell line.