A FLUORESCENCE-LC METHOD WITH NDA PRE-COLUMN DERIVATIZATION FOR FUMONISIN B₂ DETERMINATION IN BLACK ASPERGILLI CULTURES

摘要

The recent discovery that Aspergillus niger can produce fumonisins raises a new mycotoxigenic risk that requires additional studies to assess the impact on agricultural commodities. Here, we report the development and validation of a sensitive LC-fluorescence method for the identification of fumonisin B₂ in black aspergilli cultures. The performances of naphthalene-2,3-dicarboxaldehyde (NDA), o-phthaldialdehyde (OPA), and 9-fluorenylmethyl chloroformate (FMOC) for the precolumn derivatization of samples were evaluated and compared. The best methodology included the following elements: NDA was used during pre-column derivatization; the derivatives were separated by a 30 min isocratic elution with acetonitrile/H₂O/acetic acid (60:40:1, v/v/v), pumped at a flow rate of 1 mL · min−1 on a polar C₁₈ reversed-phase YMC-Pack ODS-AQ column (250 × 4.6 mm, 5 µm); and fluorescence detection using excitation and emission wavelengths of 420 and 500 nm, respectively. The calibration curve was found to give a good linear response (r2  0.9999) from 1 to 200 µg · L−1. For FB₂, which is the main fumonisin detected in black aspergilli thus far, this method measured an LOD and an LOQ of 2 and 6 µg · kg−1, respectively. Using this method, the amount of FB₂ in black aspergilli cultures can be simply and rapidly determined.View full textDownload full textKeywords Aspergillus niger , black aspergilli, fluorescence detection, fumonisin B2 , HPLC, Naphthalene-2,3-dicarboxaldehydeRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.575982