ENANTIOSELECTIVE QUANTIFICATION OF DOXYLAMINE IN HUMAN PLASMA BY HPLC

摘要

A high pressure liquid chromatography-diode array detector (HPLC-DAD) method using amylose tris(3,5-dimethylphenyl carbamate) chiral stationary phase (Chiralpak AD-H) is described for the determination of doxylamine enantiomers in human plasma. Doxylamine enantiomers were separated on a Chiralpak AD-H column using a mobile phase composed of n-hexane-2-propanol-diethylamine (98:2:0.025, v/v/v). Diphenhydramine was used as an internal Standard (IS). Doxylamine was extracted from plasma samples using dichloromethane:hexane (1:2 v/v), which yielded high extraction yields (87%), satisfactory precision (RSD  1.05%), and good selectivity. Linearity was found in the 8-40 µg · mL−1 range with the limits of detection 0.13 µg · mL−1. Doxylamine enantiomers were well separated with no interference from endogenous plasma constituents. The method developed, showed a good linearity, sensitivity, and repeatability.View full textDownload full textKeywordsamylose tris(3,5-dimethylphenyl carbamate), doxylamine, enantiomeric analysis, HPLC, pharmaceutical analysisRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.560700