Effect of Ginsenoside-Rb_1 on Cardiomyocyte Apoptosis after Ischemia and Reperfusion in Rats

摘要

The effect of ginsenoside Rb_1 on cardiomyocyte apotosis after ischemia (30 min) and reperfusion (6 h) in rats was observed. The ischemia/ reperfusion heart model was established by ligating left anterior descending branch of coronary artery in Wistar rats. The apoptotic cardiomy-ocytes were examined under transmission electron microscopy and counted by in situ nick end la-beling (TUNED method and light microscopy. Results showed that (1) The apoptotic cardiomyocytes were found in ischemic regions in the ischemia/reperfusion group, but not in the sham-operating group under transmission electron microscopy; (2) The number of apoptotic cells were 134. 45+-45. 61/field in the ischemia/reperfusion group, 0/field in the sham-operating group and 51. 65+-13. 71/field in the ginsenoside Rb_1-treated group. The differences were significant among the three groups (P<0. 01). It was concluded that myocardial ischemia-reperfusion could induce cardiomyocyte apoptosis, and ginsenoside Rb_1 could significantly inhibit cardiomyocyte apoptosis induced by ischemia-reperfusion in rats, indicating that ginsenoside Rb_1 could inhibit cardiomyocyte apoptosis induced by ischemia-reperfusion, thus alleviating ischemia-reperfusion injury.