Effects of Serum from Aplastic Anemia patients on the Expression of Cyclin D3 Isoform in Umbilical Cord Blood CD34~+ Cells

摘要

The pathogenesis of aplastic anemia (A A) was explored and the effects of A A serum on the expression of crucial cyclin D isoform (cyclin D3) in umbilical cord blood hematopoietic stem/ progenitor cells were observed. The CD34~+ cells were isolated from the cord blood with MIDI-MACS Semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; The expression level of cyclin D3 was assayed by semi-quantitative RT-PCR and Western-blot after the hematopoietic stem/progenitor cells were incubated in AA serum. The results showed that the AA serum could inhibit the formation of CFU-GM and down regulate the expression level of the cyclin D3 at the mRNA and protein level respectively. In conclusion, the A A serum could inhibit the proliferation of hematopoietic stem cells and down regulate level of cyclin D3 , which might be one mechanism of hematopoiesis inhibition in AA.