Effect of Wild-type p53 Gene Transfection on the Growth and Radiotherapeutic Sensitivity of Human Glioma Cells

摘要

To evaluate the effect of wild-type p53 gene on the growth and radiotherapeutic sensitivity of human glioma cells, plasmid PC53-SN3 carrying wild-type p53 gene was transfected into U251 cells. p53 gene expression in transfected cells was detected by RT-PCR, and the cell growth inhibition and apoptosis in the absence or presence of irradiation were assessed by MTT and flow cytome-try. The transfection of p53 gene into U251 cells was confirmed by RT-PCR. MTT showed that p53 gene alone induced strong inhibitory effect on the growth of U251 cells (inhibition rate (IR): (79.60 ± 5.69) %). The killing effect of irradiation alone on U251 cells was not strong (IR: (17.06 ± 4.35) %. (17.39 ± 1.67) %, (18.73 ± 4.68) %) and increased with the irradiation doses (3, 6, 9 Gy). When combined treatment of wild-type p53 gene transfection and irradiation was used, the effect was significantly increased (IR: (80.60 ± 5.35) %, (90.30 ± 1.67) %, (91.30 ± 2.01) %). The apoptosis rate of U251 cells induced by p53 gene transfection was 17.38 %. The rate induced by irradiation increased (4.61 %, 4.84 %, 5.40 %) with the irradiation doses (3, 6, 9 Gy). The apoptosis rate was also significantly increased (17.80 %, 20.03 %, 22.34 %) after combined treatment of p53 and irradiation with different doses (3, 6, 9 Gy). It is concluded that wild-type p53 gene and irradiation could result in synergistic inhibitory effect on the growth of human glioma cells.