Dual Effect of 3, 4-Dihydroxyacetophenone on LPS-induced Apoptosis in RAW264. 7 Cells by Modulating the Production of TNF-α

摘要

To explore the pharmacological effect of 3 ,4-dihydroxyacetophenone (DHAP) on the ap-optosis of RAW264. 7 macrophage cells and the mechanism, RAW264. 7 macrophage cells were treated with 100 or 500 mg/L lipopolysaccharide (LPS), with or without 10~(-5) mol/L DHAP for 24 h, Trypan blue dye exclusion assay was used to assess cell viability. 'Cell apoptosis was morphological studied and flow cytometric assay was used. Tumor necrosis factor-α (TNF-α) level was measured by ELISA methods. I_K B protein was determined by Western blotting. Our results showed that in 100 mg/L LPS-stimulated macrophages, DHAP enhanced the cell apoptosis while in 500 mg/L LPS-stimulated macrophages, DHAP significantly inhibited the cell apoptosis. In both groups, DHAP increased the level of I_K B but decreased the level of TNF-α. It is concluded that DHAP has dual effect on the apoptosis of RAW 264. 7 cells treated with different concentrations of LPS. This effect may be due to the inhibition of activation of NF-κB and autocrine production of TNFα. Our study suggests that DHAP may have anti-inflammatory effect on LPS-activated macrophages.