Genetic transformation of loblolly pine using mature zygotic embryo explants by Agrobacterium tumefaciens

摘要

Agrobacterium tumefaciens strain LBA 4404 carrying p B1121 plasmid was used to transform mature zygotic embryos of three genotypes (E-Hb, E-Ma, and E-Mc) of loblolly pine. The results demonstrated that the expression frequency of β-glucuronidase reporter gene (GUS) VARIED AMONG GENOTYPES AFTER MATURE ZYGOTIC EM- BRYOS WERE INFECTED WITH Agrobacterium tumefaciens cultures. The highest frequency (27.8/100) of GUS expressing Embryos was obtained from genotype E-Mc with mean number of 21.9 blue GUS spots per embryo.