Polyamines Affect Activity of Aminopeptidases from Lactobacillus sake

摘要

The effects of polyamines (agmatine, cadaverine, putrescino, spermidine and spermine) on the activity of the main aminopeptidases (AP I and AP II) from Lactobacillus sake were determined. Concentrations in the range of 1 mM caused 6-25/100 inhibition of AP I except for spermine (45/100 inhibition). Higher polyamine levels (5-10 mM), except for pu- trescine, exerted a stronger inhibition (20-60/100) on AP I. Agmatine and putrescine also reduced AP II activity (6-25/100) at concentrations of 0.1-10 mM while cadaverine did not have a notable effect. Spermidine and spermine stimulated (15-70/100) the activity of AP II at concentrations >1 mM. Thus, control of polyamine levels is important because they are a potential hazard for human health and also because of their effects on aminopeptidase activity.