Free Radical Scavenging, Cytotoxic, and Hemolytic Activities of an Active Antioxidant Compound Ethyl Gallate from Leaves of Acacia Nilotica (L) Wild. Ex. Delile Subsp. Indica (Benth.) Brenan

摘要

In the present study, free radical scavenging, cytotoxic, and hemolytic activities of the polyphenolic compound ethyl gallate isolated from ethanol extract of Acacia nilotica Wild. Ex. Del. leaves were determined. The free radical-scavenging activities of the ethyl gallate were demonstrated in several in vitro assays in order to evaluate the possible antioxidant mechanism. The results revealed ethyl gallate as hydrogen donor, metal chelator, and free radical scavenger. Ethyl gallate was effective in scavenging l,l-Diphenyl-2-picrylhydrazyl (DPPH) radicals and the IC_(50) value was lower than all the positive controls used in this study. Deoxyribose degradation assay revealed that ethyl gallate had more iron-chelating ability than the direct hydroxyl radical-scavenging ability. The results of the cytotoxic study revealed that the compound was moderately active and IC_(50) value was found to be >100 /Ug/mL for Vero cell lines and 72 /Ltg/mL for Hela cell lines. The compound possessed no hemolytic activity against rat and human erythrocytes revealing its cytotoxic mechanism and nontoxicity. The results from this work will provide an important information for the food and pharmacological industries with respect to the use of the compound as an antioxidant and a health-related drug.