Research Note: Thin Agar Layer Method for Recovery of Heat-Injured Listeria monocytogenes

摘要

A thin agar layer (TAL) method was developed to recover heat-injured Listeria monocytogenes. Modified Oxford medium (MOX), a selective plating medium, inhibits heat-injured L. Monocytogenes from growing, wherease tryptic soy agar 9TSA), a nonselective medium, does not, In order to facilitate recovery of heat-injured L. Monocytogenes cells while providing selectivity of isolation of L. Monocytogenes from other bacteria in the sample, a unique TAL procedure was developed by overlaying 5 ml of nonselective medium (TSA) onto prepoured and solidified MOX medium in an 8.5-cm-diameter petri dish.