Assessment of in vivo antioxidant activity of a tripeptide Ala-Tyr-Ile from Jiuzao (a by-product of baijiu distillation) protein hydrolysates and its stability in baijiu

摘要

Peptide Ala-Tyr-Ile (AYI) was isolated from Jiuzao protein hydrolysates and provedwith antioxidant ability in vitro in the previous study. Considering the limitation invitro, the antioxidant activity and potential mechanism in vivo of AYI were investigatedby AAPH-induced oxidative stress Sprague-Dawley (SD) rat model in thisstudy. The results showed that AYI could activate Nrf2/Keap1-p38/PI3K-MafK signalingpathway both on gene and protein expressions. Meanwhile, AYI could markedly(p ＜ .05) increase downstream antioxidant enzymes (i.e., CAT, GPx, SOD, and HO-1)expressions and suppress the expressions of inflammatory factors (i.e., TNF-α, IL-1β,IL-6, and NO). The results above indicated potential antioxidant and anti-inflammatoryactivity in vivo of peptide AYI. In addition, AYI could also exist stably in baijiuafter 14-day storage. This study provides a better thought for the use of Jiuzao toisolate antioxidant peptides and enhance baijiu antioxidant function by adding thesepeptides in it.