High Pressure Extraction of Astaxanthin from Shrimp Waste (Penaeus Vannamei Boone): Effect on Yield and Antioxidant Activity

摘要

High pressure extraction (HPE) of astaxanthin from shrimp (Penaeus Vannamei Boone) waste at different pressure (0.1-600 MPa) and holding times (0-20min), and with different solvents (acetone, dichloromethane and ethanol) and solvent to solid ratios (10-50mL/g) was evaluated, and extraction yields and quality of the extracts were determined. Antioxidant activity of the extract from HPE and conventional solvent extraction (at ambient pressure) were compared based on DPPH (1,1-diphenyl-2-picrylhydrazyl) and superoxide anion radical of scavenging potentials. Besides, surfaces of shrimp shells following different treatments were characterized by scanning electron microscopy. The results revealed that (1) HPE resulted in a higher extraction yield of astaxanthin and required shorter extraction times, (2) ethanol as solvent and a solvent to solid ratio of 20mL/g was a good combination for HPE for high extraction yield of astaxanthin and (3) HPE extraction resulted in a better antioxidant activity in the extract than conventional solvent extraction.