Extraction and structural characterization of collagen from fishbone by high intensity pulsed electric fields

摘要

Collagen was extracted from fishbone by high intensity pulsed electric fields (PEF) and its structure was characterized. A maximum collagen yield of 16.13 mg/mL was achieved under the following conditions: pepsin dosage of 1%, material-liquid ratio of 1:10, electric field strength of 20 kV/cm, and pulse number of 8. The change of each secondary structure component content was highly correlated with the PEF operating parameters (R-2 > .900). The tertiary structure of collagen was changed by PEF. The surface hydrophobicity increased first and then decreased with the PEF operating parameters. Scanning electron microscopy results showed the tissues changes of sample particles from dispersed and disorganized to uniform by PEF, but the high electric field strength (>30 kV/cm) and pulse number (>8) causes samples over agglomerated, which were dispersed disorderly. This article has proposed a new approach for comprehensive utilization of fishbone, which has important research significance for the promotion of PEF. Practical Applications Collagen was extracted from fishbone by high intensity pulsed electric fields. The prepared fishbone collagen can be widely used in foods, cosmetics, biology, fertilizers, medical materials, and other fields. This article provides a new technique for comprehensive utilization of fishbone. The structure of extracted collagen was characterized. This opens up a new dimension in the study of the impact of PEF technology on protein structure.