In vitro effects of metal ions on lipid peroxidation induced by alcohol in mice liver homogenate

摘要

The study is aimed to estimate the effect of different heavy metals such as Hg~(2+), Cd~(2+), Mn~(2+), Cr~(3+) Ni~(2+), Se_2O_3~(2-), As_2O_3 water solution and combined effects of Hg~(2+), Mn~(2+), Cr~(3+) on lipid peroxidation in mice liver homogenate in vitro. Lipid peroxidation was determined as thiobarbituric acid-reacting materials (TBA). We select five different concentrations of selected ions for experiments. Correlations used to identify the concentration of ions associated with lipid peroxidation. The rate of lipid peroxide formation in mice liver homogenate increased with the gradual addition of alcohol. When alcohol dose was up to 0.5 ml, the rate of lipid peroxide formation was greatest. At tested concentrations, the effects of metal ions on lipid peroxidation induced by alcohol were classified into three groups, and are as follows : (1) simulative, Hg~(2+). (2) inhibitory, Mn~(2+), Cr~(3+), Ni~(2+), Se_2O_3~(2-). (3) ambiguous, Cd~(2+), As_2O_3 water solution. When Hg~(2+), Mn~(2+) and Cr~(3+) were added to the mice liver homogenate with alcohol at the same time, Hg~(2+), Mn~(2+) were the main agents for the rate of alcohol induced lipid peroxidation. The simulative effect of Hg~(2+) on lipid peroxidation induced by alcohol indicate that alcohol-drinkers will have further health risk when they are exposed in polluted regions than others, and Mn~(2+), Cr~(3+), Ni~(2+), Se_2O_3~(2-) may act as free radical scavengers and preventive remedy for alcoholism in part. Furthermore, analysis of combined effect of Hg~(2+)Mn~(2+) and Cr~(3+) provide us a new way to estimate the combined effect of multi-materials.