Partitioning of Amino Acids Flowing to the Abomasum into Feed, Bacterial, Protozoal, and Endogenous Fractions

摘要

We partitioned the flow of amino acids (AA) to the abomasum among rumen undegradable protein (RUP) and bacterial, protozoal, and endogenous fractions us- ing four Holstein cows in midlactation that were equipped with ruminal and abomasal cannulas. A 2 x 2 factorial design with four diets, combinations of high or low ruminally degradable organic matter, and rumen degradable protein, was employed. Crude protein (CP) and AA contents of ruminal bacteria and protozoa and abomasal digesta were determined. Equations for the source compositions and in vivo flows of CP and 16 AA were then solved simultaneously with a linear program to estimate the contribution of RUP, bacterial, proto- zoal, and endogenous CP to AA flows. The flows of RUP and bacterial AA were not affected by diet. Low dietary RDP increased the flow of protozoal AA to the aboma- sum, but the ruminally degradable organic matter con- tent of the diet did not affect protozoal AA flow. Across diets, RUP, bacterial, protozoal, and endogenous frac- tions provided 55, 33, 11, and <1/100 of the CP, and 62, 26, 12, and <1/100 of the AA that reached the abomasum. The linear program was a useful tool for partitioning AA that flows to the abomasum. The technique may also allow dietary effects on ruminal microbes and the AA profile of protein flowing to the duodenum to be better understood and perhaps manipulated.