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首页> 外文期刊>Journal of dairy science >Insulin stimulates glucose uptake via a phosphatidylinositide 3-kinase-linked signaling pathway in bovine mammary epithelial cells
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Insulin stimulates glucose uptake via a phosphatidylinositide 3-kinase-linked signaling pathway in bovine mammary epithelial cells

机译:胰岛素通过牛乳腺上皮细胞中的磷脂酰肌醇3激酶相关信号通路刺激葡萄糖摄取

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摘要

The aim of this study was to investigate the effects of insulin on glucose uptake in lactating bovine mammary epithelial cells (BMEC). Primary BMEC were cultured in Dulbecco's modified Eagle's mediumutrient mixture F-12 and treated with different levels of insulin (0, 5, 50, and 500 ng/mL) for 48 h after a 24-h starvation without fetal calf serum. Compared with the control cells (0 ng of insulin/mL), cell proliferation was enhanced by insulin treatment at all tested levels. Insulin significantly increased glucose uptake at a concentration of 500 ng/mL. In addition, the protein synthesis inhibitor cycloheximide (0.5 mg/mL) counteracted the insulin-elevated glucose uptake, thereby suggesting that newly synthesized transporter protein might take part in the insulin-induced glucose uptake. Furthermore, pretreatment of the cells with SB203580, an inhibitor of p38 mitogen-activated protein kinase, did not influence the insulin-induced glucose uptake, but LY294002, a specific inhibitor of phosphatidylinositide 3-kinase, significantly reduced the insulin-stimulated glucose uptake. These results indicated that insulin-induced glucose uptake in BMEC may involve the phosphatidylinositide 3-kinase- but not mitogen-activated protein kinase-mediated signaling pathways.
机译:这项研究的目的是研究胰岛素对泌乳的牛乳腺上皮细胞(BMEC)中葡萄糖摄取的影响。将原代BMEC在Dulbecco改良的Eagle's培养基/营养混合物F-12中培养,并在无胎牛血清饥饿24小时后,用不同水平的胰岛素(0、5、50和500 ng / mL)处理48小时。与对照细胞(0 ng胰岛素/ mL)相比,在所有测试水平下通过胰岛素处理均可增强细胞增殖。胰岛素以500 ng / mL的浓度显着增加葡萄糖的摄取。此外,蛋白质合成抑制剂环己酰亚胺(0.5 mg / mL)抵消了胰岛素升高的葡萄糖摄取,从而表明新合成的转运蛋白可能参与了胰岛素诱导的葡萄糖摄取。此外,用p38促分裂原活化蛋白激酶的抑制剂SB203580预处理细胞不会影响胰岛素诱导的葡萄糖摄取,但是磷脂酰肌醇3激酶的特异性抑制剂LY294002则显着降低了胰岛素刺激的葡萄糖摄取。这些结果表明,BMEC中胰岛素诱导的葡萄糖摄取可能与磷脂酰肌醇3激酶有关,而与促分裂原活化的蛋白激酶介导的信号传导途径无关。

著录项

  • 来源
    《Journal of dairy science》 |2014年第6期|3660-3665|共6页
  • 作者单位

    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, P. R. China ,Shaanxi Institute of Zoology, 88 Xingqing Road, Xi'an 710032, P. R. China;

    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, P. R. China;

    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, P. R. China;

    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, P. R. China ,Laboratory of Lactation and Metabolic Physiology, Department of Animal Science, University of Vermont, Burlington 05405;

    Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, P. R. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bovine; insulin; glucose uptake; glucose transporter 8 (GLUT8); mammary epithelial cell;

    机译:牛胰岛素;葡萄糖摄取葡萄糖转运蛋白8(GLUT8);乳腺上皮细胞;

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