The effect of ordering of internal water in thaumatin and lysozyme crystals as revealed by Raman method

摘要

The correlation between the relative intensity of water Raman band and crystal quality was studeid for thaumatin and tetragonal lysozyme crystals grown under differnt conditions. The intensity variation of the band, revealed for the set of crystals was interpreted as being due to the different extents of ordering of internal water molecules. It was suggested that ordering is mainly relative to the alignment of the angle between the O-H bonds in water, since this angle serves as a normal coordinate for the symmetric bending vibration of H-O-H unit an dthe spectral width of the corresponding Raman band (2V_2) at 3212 cm~(-1) appeared to be the main indicator of ordering.