DNA-binding and cleavage activity of a new alkoxo and acetate-bridged dinuclear copper(II) complex

摘要

A new copper complex, [Cu₂L(OAc)(CH₃OH)] · CH₃OH, where H₃L is 1,3-bis(5-methylsalicylideneimino)propan-2-ol, has been synthesized and characterized by single-crystal X-ray diffraction, IR, ES-MS, and UV-Vis techniques. The coordination polyhedra of copper(II) can be described as a tetrahedron and a planar quadrilateral. The interaction between the complex and calf thymus DNA/DNA was investigated by UV-Vis, fluorescence, circular dichroism spectra, and viscosity. All the results show that the interaction mode of the complex with DNA is intercalative with a binding constant of 1.16 × 105 (mol L−1)−1 and linear Stern-Volmer quenching constant of 4.89 × 102 (mol L−1)−1, respectively. Agarose gel electrophoresis exhibits that the complex can transform supercoiled to nicked form and exhibits effective DNA-cleavage activity via hydrolytic-cleavage mechanism.View full textDownload full textKeywordsCrystal structure, DNA-cleavage activities, DNA-bindingRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/00958972.2012.658567