Identifying prognostic markers for multiplemyeloma through integration and analysis of MMRF-CoMMpass data

摘要

Multiple myeloma (MM) is the second most frequent haematological malignancy in the world although the related pathogenesis remains unclear. The study of how gene expression profiling (GEP) is correlated with patients? survival could be important for understanding the initiation and progression of MM. In order to aid researchers in identifying new prognostic RNA biomarkers as targets for functional cell-based studies, the use of appropriate bioinformatic tools for integrative analysis is required. In this context, TCGABiolinks package represents a valid tool for integrative analysis of MM data if its functions are properly adapted for handling MMRF data. This paper aims to extend largely our previous work [1] in which we introduced some bridging functions to make TCGABiolinks package able to deal with Multiple Myeloma Research Foundation (MMRF) CoMMpass study data available at the NCI?s Genomic Data Commons (GDC) Data Portal. Here we present an integrative analysis workflow based on the usage of a novel R-package, called MMRFBiolinks, that collects the set of the previously mentioned bridging functions besides of extending them. Our workflow leads towards a comparative analysis of MMRF data stored at GDC Data Portal that allows to carry out a Kaplan Meier (KM) Survival Analysis and an enrichment analysis for a differential gene expression (DGE) gene set. Furthermore, it leads towards an integrative analysis of MMRF Research Gateway (MMRF-RG) data. In order to show the potential of our workflow, we present two case studies. The former deals with RNA-Seq data of MM Bone Marrow sample types available at GDC Data Portal. The latter deals with MMRF-RG data for analyzing the correlation between canonical variants in a gene set obtained from the case study 1 and the treatment outcome as well as the treatment class.