A rapid scoring technique for HER2 assessment following silver in situ hybridisation is accurate, robust and reliable

摘要

Since the introduction of mandatory HERZ assessment of all newly diagnosed breast carcinomas in 2005, the debate as to the most appropriate method for this testing has continued. We, and others, have shown that chromogenic in situ hybridisation (CISH) is a robust and reliable method for HERZ assessment and is very accurate when compared with the 'gold standard' fluorescent in situ hybridisation (FISH). CISH is a single-probe brightfield gene-based assay that uses a more straightforward scoring assessment based on an evaluation of the hybridisation pattern rather than a detailed enumeration of the signals in individual tumour cells.