Reactive and neoplastic lymphocytes in human bone marrow: morphological, immunohistological, and molecular biological investigations on biopsy specimens

摘要

Background-Slight, diffuse of focal lym- phocyte proliferation is relatively com- mon in bone marrow biopsy specimens. It may be impossible to determine whether this represents a reactive lymphocytosis of low grade non-Hodgkin lymphoma (NHL) on the basis of routine investiga- tions alone. Aim-To investigate the supplementary Use of molecular biological techniques in This situation. Methods-529 formalin fixed, paraffin Embedded bone marrow biopsy specimens From the iliac crest were subjected to his- Tological and immunohistochemical Staining to determine the number and Nature of the lymphocytes present.