Complex Formation between the lumenal Domain of Adenovirus E3-19k Protein and the extracellular Domain of Class I MHC Molecule In vitro

摘要

The Ad2 E3-19k protein inhibits the transport of newly synthesized class I MHC molecules to the cell sur- face, thereby interfering with antigen presentation. The details of the interaction between E3-19k protein and class I MHC molecules have not been well-defined. In this present study, we describe the use of gel filtration HPLC for confirming the binding interaction of two domain proteins, E3-19k and MHC class I antigen, and Subsequently the characterization of protein complex by SDS-PAGE. Our results demonstrate the complex Formation between Ad2 lumenal E3-19k (108 amino acids, wt 108) and HLA-A ~*0201 molecule in vitro. Titra- Tion experiments will be employed in the future to determine stoichiometry and verify the specific interac- Tions.