Complex Formation between the lumenal Domain of Adenovirus E3-19k Protein and the extracellular Domain of Class I MHC Molecule In vitro

摘要

The Ad2 E3-19k protein inhibits the transport of newly synthesizedclass I MHC molecules to the cell sur- face, thereby interfering withantigen presentation. The details of the interaction between E3-19kprotein and class I MHC molecules have not been well-defined. In thispresent study, we describe the use of gel filtration HPLC forconfirming the binding interaction of two domain proteins, E3-19k andMHC class I antigen, and Subsequently the characterization of proteincomplex by SDS-PAGE. Our results demonstrate the complex Formationbetween Ad2 lumenal E3-19k (108 amino acids, wt 108) and HLA-A ~*0201molecule in vitro. Titra- Tion experiments will be employed in thefuture to determine stoichiometry and verify the specific interac- Tions.