Fingerprints of Phalaenopsis Tissues in Growth and Spike Induction Periods-A Solid-state ~(13)C NMR Approach

摘要

Solid-state Nuclear Magnetic Resonance (ss-NMR) ~(13)C single-pulse excitation spectroscopy in combination with the magic-angle spinning (MAS) technique was applied to a series of Phalaenopsis tissues, including the leaf, sheath, stem, and root, at different growth and spiking periods. Compared with {~1H}/~(13)C cross-polarization MAS spectra, the ~(13)C single-pulse excitation MAS spectra displayed very distinct spectral patterns, recognizable as fingerprints of the tissues studied. ~1Here, we demonstrate that solid-state ~(13)C single-pulse excitation NMR spectroscopy provides a direct and robust analytical tool for studying the various tissues of Phalaenopsis in different growth and spiking induction periods.