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Quantitative mapping of collagen fiber alignment in thick tissue samples using transmission polarized-light microscopy

机译:使用透射偏振光显微镜对厚组织样品中胶原纤维排列的定量定位

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摘要

Immersion optical clearing makes it possible to use transmission polarized-light microscopy for characterization of thick (200 to 2000 μm) layers of biological tissues. We discuss polarization properties of thick samples in the context of the problem of characterization of collagen fiber alignment in connective tissues such as sclera and dermis. Optical chirality caused by azimuthal variations of the macroscopic (effective) optic axis of the medium across the sample thickness should be considered in polarization mapping of thick samples of these tissues. We experimentally evaluate to what extent the optical chirality affects the measurement results in typical situations and show under what conditions it can be easily taken into account and does not hinder, but rather helps, in characterization of collagen fiber alignment.
机译:浸没式光学清洁技术使得可以使用透射偏振光显微镜来表征厚(200至2000μm)的生物组织层。我们讨论了结缔组织(如巩膜和真皮)中胶原纤维排列的表征问题,讨论了厚样品的极化特性。在这些组织的厚样本的偏振图中,应考虑由介质的宏观(有效)光轴在整个样本厚度上的方位角变化引起的光学手性。我们通过实验评估了光学手征性在典型情况下对测量结果的影响程度,并显示了在什么条件下可以很容易地考虑到它,并且不会阻碍而是有助于表征胶原纤维。

著录项

  • 来源
    《Journal of biomedical optics》 |2016年第7期|071111.1-071111.12|共12页
  • 作者单位

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

    Saratov State University, Faculty of Physics, Department of Optics and Biophotonics, Astrakhanskaya 83, Saratov 410012, Russia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    collagen fiber alignment; polarization mapping; microscopy; sclera; dermis;

    机译:胶原纤维排列;极化映射;显微镜巩膜;真皮;

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