Optimisation and validation of a fluorescence polarisation immunoassay for rapid detection of zearalenone in corn

摘要

The purpose of this study was to develop a highly sensitive fluorescence polarisation immunoassay (FPI A) for the detection of zearalenone (ZEN). The method was optimised and validated to examine the feasibility of performing FPIA using several tracers synthesised from different fluorescence labels and chemicals structurally mimicking ZEN. Optimum extraction conditions were determined, and 4-(aminomethyl) fluorescein-labelled ZEN tracer (ZEN-4AMF) was selected as the tracer. None of the tracers mimicking ZEN gave a favourable response with the monoclonal anti-ZEN antibody. When tested on a corn matrix, the FPIA showed a detection limit of 77 μg kg~(-1) within 3 min, excluding extraction time. Recovery of ZEN averaged 101% (intraday) and 109% (interday), and trueness averaged 111%. Ruggedness was satisfactory but cross-reactivity with ZEN analogues was relatively high. These results suggest that the current FPIA for the detection of ZEN has a potential as an easy and rapid screening tool for ZEN and its analogues in corn.