Manipulation of volatile compound transformation in durian wine by nitrogen supplementation

摘要

This study evaluated the impact of added diammonium phosphate (DAP) and a L-leucine-L-phenylala-nine mixture (Leu+Phe) on durian wine fermentation by Saccharomyces cerevisiae var. bayanus EC-1118. Changes in yeast cell population, °Brix, sugars, organic acids and pH were similar in all fermentations, regardless of nitrogen supplementation at a concentration of 150 mg N L~(-1). The supplementation of Leu +Phe accelerated the utilisation and reduced the formation of volatile sulphur compounds such as diethyl disulphide, ethyl thioacetate and 2-(ethylthio)ethanol, which were 64.3%, 26.0% and 48.4% lower than the control, respectively. Nevertheless, the supplementation of Leu+Phe heightened the production of iso-amyl alcohol, 2-phenylethyl alcohol and their corresponding esters, especially isoamyl acetate was 1.6 times and 2-phenylethyl acetate was 26.5 times higher than the control. The supplementation with DAP exerted negligible effects on the volatile composition. The results of this study suggest that the addition of specific amino acids may be a novel approach to manipulating durian wine flavour by suppressing or accentuating the formation of certain aroma compounds.