In vitro antibacterial activity of leaf extracts of Rhizophora mucronata L. against multi drug resistant Vibrio spp. isolated from marine water Lobster's larvae hatcheries

摘要

Present article consists the in vitro antibacterial study carried out using leaf extracts of Rhizophora mucronata L., a mangrove plant. Extracts were screened for antibacterial activity against multi-drug resistant Vibrio harveyi and Vibrio campbellii. The same were isolated from Lobster's larvae hatchery water. Antibiotic resistance and sensitivity of the isolated Vibrio spp. were tested with Hi-Media antibiotic disc. Out of total eight antibiotics discs used, V. harveyi was found highly resistant to ampicillin, oxacillin, cephalothin, vancomycin, erythromycin, and clidamycin, while, it was sensitive towards chloramphenicol (19 mm) and gentamycin (9 mm). Whereas, Vibrio campbellii was found highly resistant to ampicillin, oxacillin, cephalothin, vancomycin, and erythromycin. Vibrio campbellii was highly sensitive to chloramphenicol (20 mm), and moderately sensitive to clindamycin (9 mm), and gentamycin (14 mm). Chloroform leaf extract of Rhizophora mucronata L. was found to be the most active one against both Vibrio spp., while, hexane, ethyl acetate, and methanol extract revealed moderate activity against both Vibrio spp. However, distilled water extract did not have any activity. Chloromphenicol from the Hi-Media antibiotic and chloroform extract from Rhizophora mucronota leaves can be used as a source of alternative compound to control the Vibrio spp. which infects the Lobster's larvae.