Production of alpha amylase by salt - Tolerant Actinomycete Streptomyces sp-SBU3 isolated from marine sponge

摘要

Marine environment harbors a number of macro and microorganisms that have specific metabolic activities. Marine actinomycete Streptomyces sp. - SBU3 was isolated from marine sponge Ircinia sp. from Cape Comorin coast (Lat.8 degrees 21' N and Long 77 degrees 30'E), India for amylase activity. The effects of salinity, temperature, pH, carbon sources, nitrogen sources and incubation period were tested for alpha amylase production by the isolate. Highest enzyme activity (200 IU/ml) was obtained when the medium supplemented with 1% Nacl (w/v), glucose as a carbon source at 1 % (w/v) and casamino acid as a nitrogen source 1 % (w/v). Optimal alpha amylase production by Streptomyces sp. - SBU3 was observed in pH 7.5 at 30 degrees C during 96h of incubation period. So for no sponge associated actinomycetes have been explored for media optimization for their alpha amylase production.