Selective cell killing by microparticle absorption of pulsed laserradiation

摘要

The mechanism of interaction between subcellular pigmentnmicroparticles and short pulse laser radiation was investigated withnnanosecond time-resolved microscopy and fluorescence microscopy.nStroboscopic illumination was used to capture images of transient eventsninduced in cells by laser pulses. Fluorescence microscopy enablednassessment of cell damage using fluorescent probes. Short-livednintracellular cavitation bubbles were directly visualized withinnnanoseconds after laser irradiation. Microbubble expansion and implosionntook place on the timescale of 0.1-1 Μs and were confined entirelynwithin pigmented cells with remarkable selectivity. Cells containingnparticles underwent cavitation and rapidly lost viability, whilenadjacent cells without particles remained viable. Nonpigmented cells cannbe targeted by labeling them with absorbing particles. Laser-pumpednmicroparticles provide an efficient means of selective cell targeting