Formation and relevance of 5-hydroxymethylfurfural in bioactive subcritical water extracts from olive leaves

摘要

Although subcritical water extraction (SWE) has already shown its great potential for the attainment of natural bioactive extracts, concerns still remain on possible unexpected reactions that can arise during the extraction process, usually taking place at high pressure and temperature. It is already well-known that different components might be formed during the SWE extraction protocol due e.g. to Maillard reaction, which can improve the bioactivity of the obtained extracts. On the other hand, the formation of other compounds derived from these reactions, such as 5-hydroxymethylfurfural (HMF), has raised some concerns, mainly related to its safety. In this work, the formation of HMF during subcritical water extraction, at different conditions, from olive leaves has been monitored by using liquid chromatography with mass spectrometry (LC-MS) and diode array detection (LC-DAD). The possible influence of this compound in the overall antiox-idant and antiproliferative activities against colon cancer cells has been also studied. Results showed an increase of HMF formation when increasing the extraction temperature, being the maximum concentration achieved at 200 ℃ (3.17 ug HMF/mg extract); nevertheless, the HMF contained in the olive leave extracts did not influence the antioxidant capacity or the antiproliferative activity of the natural extracts, thus demonstrating the safety of the SWE process.