Changes of Hepatic Xenobiotic-metabolizing and Antioxidant Enzyme Systems Following Endotoxin Exposure in Mice Fed with Casein or Gluten Diet

摘要

Male weanling ICR mice were fed diets composed of milk-casein or wheat-gluten at a 20/100 protein level. After being fed for 2 weeks, the mice were injected with 0.5μg/mouse endotoxin, lipopolysaccharide (LPS) from E coli, or an equivalent volume of normal saline. The animals were euthanized by decapitation at 24 hours post injection, and hepatic tissues were removed and processed to assess various enzyme activities. The endotoxin LPS injection reduced P450 content and GST activities toward 3,4-dichloronitrobenzene and trans-4-phenyl-3-buten-2-one, while the treatment raised GSSG-R activity, in mice fed the casein diet. When LPS was injected into animals fed the gluten diet, only the P450 content was lowered. These results suggest that a drastic loss of hepatic P450 caused by the endotoxin LPS under conditions of protein malnutrition may have a harmful effect on various biological systems.