LC/MS/MSによるヒト血清・尿中のヒヨスチアミンおよびスコポラミンの分析

摘要

A simple method was developed for the analysis of hyoscyamine and scopolamine in human serum and urine using liquid chromatography with tandem mass spectrometry (LC/MS/MS). Hyoscyamine and scopolamine in serum and urine were cleaned up with an Oasis HLB cartridge and a PSA cartridge. The LC separation was carried out on an ODS column, using linear gradient elution with 5 mmol/L IPCC-MS3-methanol as the mobile phase. The mass spectral acquisition was done in the positive ion mode by applying selected reaction monitoring (SRM). The recoveries of hyoscyamine and scopolamine were 86.0-105% from human serum and urine fortified at 0.2 ng/mL and 10 ng/mL. The detection limits of hyoscyamine and scopolamine were 0.02 ng/mL. Four serum and three urine samples of humans poisoned by eating Datura innoxia Mill. were analyzed by this method. Hyoscyamine and scopolamine were detected at the levels of 0.45-3.5 ng/mL in all serum samples and 170-670 ng/mL in all urine samples.%ヒヨスチアミンおよびスコポラミン（Fig.1）はチョウセrnンアサガオ属やハシリドコロ属などのナス科植物に含まれrnるトロパンアルカロイドであり，摂取すると呼吸困難，意rn識混濁，幻覚などの症状を呈し，大量に摂取すると死亡すrnることもある．これらの植物の誤食による食中毒事件rnは全国で毎年発生しており，有毒植物によるものの中で発rn生件数が多い.