農産物中ベンジルアミノプリン分析法

摘要

ベンジルアミノプリンは，植物ホルモンの細胞分裂誘導rn物質（サイトカイニン）頬緑化合物として合成された植物rn成長調整剤で，同じサイトカイニン類縁化合物であるカイrnネチンと同様，アミノプリン誘導体物質である．この物質rnは食品衛生法に基づ〈残留農薬基準（暫定基準）が設定さrnれているが，その試験法に関しては，旧作物残留に係る登rn録保留基準試験法（平成18年5月29日付けで廃止）にrnアルカリ熱イオン化検出器付き，あるいは高感度窒素・リrnン検出器付きガスクロマトグラフ（GC/FTD,GC/NPD）でrn分析する方法が規定されているのみであった．%A simplified method for the determination of benzylaminopurine in agricultural products by LC/MS was investigated. Benzylaminopurine in agricultural products was extracted with acetone and the extract was concentrated to below 30 mL. Buffer solution of pH 9.0 and ethyl acetate were added to the residue, and the solution was shaken. The ethyl acetate layer was evaporated to dryness, and the residue was dissolved in acetone-w-hexane (1 : 1). The solution was applied to a SAX/PSA mini-column, which was then rinsed with acetone-w-hexane (1 : 1). Benzylaminopurine was eluted with acetone-n-hexane (1:1) containing 1% water. The eluates were evaporated to dryness, and the residue was dissolved in 10 mmol/L ammonium acetate-methanol (1 : 1). Benzylaminopurine was analyzed by LC/MS. The MS detection was performed in the selected ion monitoring (SIM) mode, with detection of the M + H~+ ion of benzylaminopurine (m/z 226) generated by electrospray ionization (ESI). Recoveries of benzylaminopurine from 15 agricultural products were in the range of 83.1-97.2%. The limits of detection (S/N=3) of benzylaminopurine in all samples except green tea and in green tea were 0.0003 and 0.0012 μg/g, respectively.