HPLC-FLおよびLC/MS/MSによる食品中のオクラトキシンA,Bおよびシトリニンの同時分析法

摘要

オクラトキシン（OT）は，Aspergilluss属とPenicillium属の数種の菌により産生される腎毒性を有するカビ毒である．10種類以上あるOTの同族体のうち最も食品汚染報告が最も多いのは，オクラトキシンA（OTA）であり，次いでオクラトキシンB（OTB）である.%Methods using high-performance liquid chromatography with fluorescence detection (HPLC-FL) and using liquid chromatography with tandem mass spectrometry (LC/MS/MS) were developed for simultaneous determination of ochratoxin A (OTA), ochratoxin B (OTB) and citrinin (CIT) in cereal, fruit, and coffee products. The samples were extracted with ethyl acetate under an acidic condition, and then cleaned up with liquid-liquid separation. The test solutions were analyzed by reverse-phase HPLC-FL and LC/MS/MS. Mass spectral acquisition was performed in positive ion mode by applying multiple reaction monitoring. The performances of both detectors were almost equivalent. The recoveries of OTA and OTB were 87-111%, and that of CIT were 70-88%. The limits of quantification (S/N ≧ 10) of OTA, OTB and CIT was 0.1 μg/kg or less. These methods were considered to be useful for the determination of the three mycotoxins at low levels (0.1 μg/kg).