HPLCおよびLC-MS/MSによる加工食品中の超高甘味度甘味料アドバンテーム定量法

摘要

A simple method using HPLC and LC-MS/MS was developed for the determination of ultra-high-intensity sweetener, advantame, in processed foods. Advantame was extracted by dialysis, and cleaned up on a Sep-Pak Plus C18 cartridge, then determined by HPLC and LC-MS/MS. The recoveries from 5 kinds of processed foods fortified at the levels of 0.001 g/kg and 0.01 g/kg were 64.1-89.9% (RSD 0.9-6.9%) by HPLC and 68.8-99.9% (RSD 0.8-4.9%) by LC-MS/MS. The quantitation limit was 0.0004 g/kg by HPLC and 0.00004 g/kg by LC-MS/MS.%HPLCおよびLC-MS/MSを用いた食品中の超高甘味度甘味料であるアドバンテームの定量法を検討した.アドバンテームを透析抽出後，Sep-Pak Plus C18で精製し，HPLCおよびLC-MS/MSで定量した.5種類の市販加工食品にアドバンテームを0.001 g/kgおよび0.01 g/kg添加して回収試験を行った結果，HPLC測定では回収率は64.1〜89.9%, RSDは0.9〜6.9%, LC-MS/MS測定では回収率は68.8〜99.9%, RSDは0.8〜4.9%であった.定量限界は試料当たりHPLC分析では0.0004 g/kg, LC-MS/MS分析では0.00004g/kgであった.