Characterization of the seed proteins of velvet bean (Mucuna pruriens) from Nigria

摘要

This paper reports the preliminary characterization of seed protein fractions from seven varieties bans (Mucuna pruriens) grown in Nigeria, using soidum dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Three of the most abundant polypeptides, with approximate Mr of 23, 26 and 30 kDa, respectively, were further separated by preparative native-PAGE. N-terminal sequencing revealed the presence of the consensus sequence DDREPV-DT-PL that is also present in the soybean K unitz-type trypsin inhibitor. The albumin fraction was also shown to contain both trypsin and chymotrypsin inhibitors thorugh enzyme inhibitor assays.