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Formation mechanism of the oolong tea characteristic aroma during bruising and withering treatment

机译:乌青茶枯萎处理过程中乌龙茶特有香气的形成机理

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摘要

To elucidate formation mechanism of oolong tea aroma, the released and remaining volatiles during bruising and withering treatment were analyzed using head space solid-phase microextraction/gas chromatography-mass spectrometry. An increase in proportion of the released terpenoid volatiles (TVs) along with a decrease in proportion of the released C6green leaf volatiles (GLVs) was observed in both cultivars ‘Zhejiang139’ and ‘Foshou’. Proportion of remaining TVs also fluctuated reversely with GLVs although the level of these volatiles increased remarkably. High ratio of TVs to GLVs was the key chemical foundation of oolong tea characteristic aroma and could be regarded as a good indicator in screening cultivar for suitably producing high quality oolong tea. Combining with transcriptome analysis, increased TVs and GLVs during the treatment might be largely generated throughde novosynthesis and modulated at transcript level through up-regulation of genes involved in terpenoids metabolism and enzymatic cleavage of long-chain fatty acids.
机译:为了阐明乌龙茶香气的形成机理,采用顶空固相微萃取/气相色谱-质谱法分析了青紫和枯萎处理过程中释放的和残留的挥发物。在“浙江139”和“抚寿”两个品种中均观察到萜类挥发物(TVs)释放比例增加,而C6绿叶挥发物(GLVs)释放比例下降。剩余电视的比例也随GLV反向波动,尽管这些挥发物的水平显着增加。高TVs / GLVs比率是乌龙茶特有香气的关键化学基础,可作为筛选适宜生产优质乌龙茶品种的良好指标。结合转录组分析,治疗期间增加的TV和GLV可能主要通过新合成产生,并通过上调参与类萜代谢和长链脂肪酸的酶促裂解的基因而在转录水平上进行调节。

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