Determination of 1-phenylazo-2-naphthol (Sudan Ⅰ) in chilli powder and in chilli-containing food products by GPC clean-up and HPLC with LC/MS confirmation

摘要

A simple and rapid method is reported for the routine determination 1-phenylazo-2-naphthol (Sudan Ⅰ) in chilli powder and in chilli-containing food products. The method involved Soxtec extraction from the products followed by high-pressure gel permeation chromatographic clean-up collecting the appropriate fraction. Analysis of this fraction was by HPLC with UV/VIS detection. The limit of detection was 7 μg kg~(-1) and the limit of quantification was 13 μg kg~(-1) . The identity of Sudan Ⅰ in food products was established by electrospray LC/MS with MS/MS confirmation. From a small survey of 30 retail samples, 11 samples of crushed chilli, Italian pasta, chilli-snack and vegetable sauce contained levels of Sudan Ⅰ ranging from 24 to 5591 μg kg~(-1).