Toponomics and neurotoponomics: a new way to medical systems biology

摘要

The fluorescence robot imaging technology multi-epitope-ligand-cartography/toponomenimaging system has revolutionized the field of proteomics/functional genomics, because itnenables the investigator to locate and decipher functional protein networks, the toponome,nconsisting of hundreds of different proteins in a single cell or tissue section. The technologynhas been proven to solve key problems in biology and therapy research. It has uncovered annew cellular transdifferentiation mechanism of vascular cells giving rise to myogenic cellsnin situ and in vivo; a finding that has led to efficient cell therapy models of muscle disorders,nand discovered a new target protein in sporadic amyotrophic lateral sclerosis by hierarchicalnprotein network analysis, a finding that has been confirmed by a mouse knockout model. Anlead target protein in tumor cells that controls cell polarization as a mechanism that isnfundamental for migration and metastasis formation has also been uncovered, and newnfunctional territories in the CNS defined by high-dimensional synaptic protein clusters havenbeen unveiled. The technology can be effectively interlocked with genomics and proteomics tonoptimize time-to-market and the overall attrition rate of new drugs. This review outlines majornproofs of principle with an emphasis on neurotoponomics.