12. VIROLOGY

摘要

A procedure based on BIA Separations CIM DEAE anion-ex-change chromatography was developed to separate double-stranded (ds) RNA of hypovirus infecting phytopathogenic fungus Cry-phonectria parasitica. Using a linear gradient of 25 mM 4-morpholinepropanesulfonic acid (MOPS), pH 7.0 as a binding buffer, and 25 mM MOPS, 1.5 M NaCl, 0.1 mM EDTA, 15% iso-propanol (v/v), pH 7.0 as an elun'on buffer, hypoviral dsRNA was additionally purified from nucleic acid species present in preparations partially purified by standard CF-11 cellulose chromatography. Moreover, crude phenol/chloroform extracts of the fungal tissue were also applied to monolithic supports and CIM DEAE chromato-grams revealed clear evidence for hypoviral presence without CF-11 chromatography, nucleic acid precipitation, and electrophoresis.