Intergeneric hybridization and relationship of genera within the tribe Anthemideae Cass. (I. Dendranthema crassum (kitam.) kitam. × Crossostephium chinense (L.) Makino)

摘要

An intergeneric cross has been made between Dendranthema crassum (kitam.) kitam. (2n = 90; ♀) and Crossostephium chinense (L.) Makino (2n = 18; ♂). Most of the hybrid embryos aborted at an early developmental stage. Using ovule rescue, it was possible to establish a single intergeneric hybrid plant showing 2n = 54 chromosomes. The leaf length, leaf width and epidermal hair density of the hybrid were all intermediate between those of the parents. However the flower diameter, number of tubular florets, epidermal hair height and epidermal hair length exceeded those of both parents. A genomic in situ hybridization approach was able to distinguish between the parental genomes in the hybrid plant. Keywords Dendranthema crassum (kitam.) kitam. - Fluorescent genomic in situ hybridization - Crossostephium chinense (L.) Makino - Intergeneric cross - Anthemideae - Chrysanthemum sensu lato