Evaluation of the immunotoxicity of chlorothalonil to striped bass phagocytes following in vitro exposures

摘要

The fungicide chlorothalonil (TCIN), with both agricultural and horticultural applications, is a common aquatic pollutant. The immunotoxic potential of TCIN was investigated following a 20-h in vitor exposure of striped bass 9Moone saxatilus) Macropages to a range of sublethal concentrations. Cells from the anterior kidney were separated by density-gradient centrifugation To obtain a macrophage-enriched cell population. Following TCIN exposure, reactive oxygen species (ROS) production was evaluated Using luminol-augmented chemiluminescence; phagocytic capacity was measured by the ingetion of fluorescein isothiocyanate- Conjugated yeast; and NADPH production was estimated by monitoring the reduction of a water-soluble tetrazolium, 3-(4,5- Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-suflophenyl)-2H-tetrazolium, inner salt.